Sensitive measurement of total protein phosphorylation level in complex protein samples

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Sensitive measurement of total protein phosphorylation level in complex protein samples.

Measurement of protein phosphorylation plays an essential role in delineating cell signaling pathways. Although the detection of a specific phosphoprotein has been largely accomplished by immunological methods, a specific and sensitive assay to measure total protein phosphorylation level in complex samples such as whole cell extracts has yet to be established. Here, we present a sensitive phosp...

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ژورنال

عنوان ژورنال: The Analyst

سال: 2015

ISSN: 0003-2654,1364-5528

DOI: 10.1039/c5an00365b